Difference between revisions of "Part:BBa K1943019:Design"
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Latest revision as of 03:29, 22 October 2016
TetOn
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 857
Design Notes
This TetOn expression system contains a SV40 promoter, a puro anti-antibiotics gene, 2A and TetOn 3G promoter. We design primers and do PCR of this TetOn expression system and ligate it to pSB1C3 backbone.
Source
The plasmid was from our instructor, Huangwei's lab. And we design primers to copy down by PCR.