Difference between revisions of "Part:BBa K1896010"
 
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This part constitutively expresses [[Part:BBa_K1896001|mGFPuv2]], a monomeric GFP variant optimised for excitation by UV light (360-400nm).
 
This part constitutively expresses [[Part:BBa_K1896001|mGFPuv2]], a monomeric GFP variant optimised for excitation by UV light (360-400nm).
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The J23119 promoter is a strong consensus promoter and expression is high enough for colonies to also display visible fluorescence in daylight.
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The J23119 promoter is a strong consensus promoter and expression is high enough for colonies to display visible fluorescence in daylight.
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+
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[[File:MGFPuv2_colonies.jpeg|250px|thumb|center|Colonies of <i>E. coli</i> containing BBa_K1896010 imaged under long-wave UV light.]]
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<gallery mode="packed" heights=300px>
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File:BBa_K1896010_day.jpeg|Colonies of <i>E. coli</i> containing BBa_K1896010.
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File:BBa_K1896010_uv.jpeg|The same colonies imaged under long-wave UV light.
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</gallery>
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 21:55, 21 October 2016


mGFPuv2 generator

This part constitutively expresses mGFPuv2, a monomeric GFP variant optimised for excitation by UV light (360-400nm). The J23119 promoter is a strong consensus promoter and expression is high enough for colonies to display visible fluorescence in daylight.

  • Colonies of E. coli containing BBa_K1896010.

  • The same colonies imaged under long-wave UV light.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]