Difference between revisions of "Part:BBa I746200:Design"
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The mutants were developed by Dr Salete Newton working in Professor P. Klebba's lab at the university of Oklahoma. They were kindly sent to us by Professor Klebba. The main paper reference is: | The mutants were developed by Dr Salete Newton working in Professor P. Klebba's lab at the university of Oklahoma. They were kindly sent to us by Professor Klebba. The main paper reference is: | ||
| − | Effect of loop deletions on the binding and transport of ferric enterobactin by FepA. | + | ''Effect of loop deletions on the binding and transport of ferric enterobactin by FepA.'' |
| − | Mol Microbiol. 1999 Jun;32(6):1153-65. - Newton SM, Igo JD, Scott DC, Klebba PE. | + | ''Mol Microbiol. 1999 Jun;32(6):1153-65. - Newton SM, Igo JD, Scott DC, Klebba PE.'' |
===References=== | ===References=== | ||
Revision as of 10:53, 8 September 2007
FepA L8T Mutant - Large Diffusion pore for E. coli outer membrane.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 238
Illegal AgeI site found at 604
Illegal AgeI site found at 1231
Illegal AgeI site found at 1749 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The original mutants had an EcoR1 restriction site within their coding sequence. This had to be removed via site directed mutagenesis, using a megaprimer based method.
Source
The mutants were developed by Dr Salete Newton working in Professor P. Klebba's lab at the university of Oklahoma. They were kindly sent to us by Professor Klebba. The main paper reference is:
Effect of loop deletions on the binding and transport of ferric enterobactin by FepA. Mol Microbiol. 1999 Jun;32(6):1153-65. - Newton SM, Igo JD, Scott DC, Klebba PE.