Difference between revisions of "Part:BBa I13556:Experience"
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how you used this part and how it worked out.
 
  
 
==Transfer curve==
 
==Transfer curve==

Revision as of 21:22, 6 September 2007


Transfer curve

Protocol

  1. Two colonies of pSB4K5.I13556 were grown up in 5ml LB Kan overnight
  2. Cultures were diluted 1:1000 and grown for 2 hours in 4ml EZ Rich medium + Kan
  3. 3 replicates of 100μl aliquots each for 8 AHL concentrations were made
  4. added 100μl EZ+AHL to 8 final concentrations of AHL: 0, 1E-11, 1E-10, 1E-9, 1E-8, 1E-7, 1E-6, 1E-5 M
  5. The cells were measured on a Victor3 fluorescence plate reader and grown overnight in the plate reader at 37C

Data

All data:

I13556-fluorescence.png

I13556-OD.png

Taking the last time point for all samples, we get the following transfer curve (note that the first time point labeled as 1E-12 is actually 0 AHL and was just set there for ease of plotting):

I13556-transfercurve.png

User Reviews

UNIQ189c70cd055aba15-partinfo-00000000-QINU UNIQ189c70cd055aba15-partinfo-00000001-QINU