Difference between revisions of "Part:BBa K1976002"
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| + | <h1>mVenus with a LVA degradation tag</h1> | ||
| + | This part contains an E. coli optimized coding sequence of the yellow fluorescent protein mVenus with an associated LVA degradation tag. | ||
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| − | < | + | ===<h2>Usage</h2>=== |
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| + | In some cases a fast signaling reporter, as well as a fast decay of the said reporter is necessary to create a specific genetic circuit. One of the best fitting reporters might be the <i>E.coli</i> optimized version of mVenus. With an average maturation time of 40 min in vitro it is faster than GFP. In order to prevent a persistent fluorescence after the expression of the reporter stopped, mVenus is expressed with a LVA degradation tag to decrease the protein half-life. Another positive aspect of mVenus is the lowered sensitivity towards pH and chloride ion concentration, one of the drawbacks of wild-type GFP. The lack of disulfide bonds enables fluorescence under reductive conditions. Moreover, the reporter is not regulated by any proteins, cofactors or substrates. Therefore mVenus does not only expand the spectrum of fluorescence proteins in the registry, it also is a good alternative for various genetic circuits. | ||
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| − | + | ===<h2>Characteristica</h2>=== | |
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| − | + | ===<h2>Sequence and Features</h2>=== | |
| + | <partinfo>BBA_K1976002 SequenceAndFeatures</partinfo> | ||
Revision as of 18:03, 21 October 2016
mVenus with a LVA degradation tag
This part contains an E. coli optimized coding sequence of the yellow fluorescent protein mVenus with an associated LVA degradation tag.Usage
In some cases a fast signaling reporter, as well as a fast decay of the said reporter is necessary to create a specific genetic circuit. One of the best fitting reporters might be the E.coli optimized version of mVenus. With an average maturation time of 40 min in vitro it is faster than GFP. In order to prevent a persistent fluorescence after the expression of the reporter stopped, mVenus is expressed with a LVA degradation tag to decrease the protein half-life. Another positive aspect of mVenus is the lowered sensitivity towards pH and chloride ion concentration, one of the drawbacks of wild-type GFP. The lack of disulfide bonds enables fluorescence under reductive conditions. Moreover, the reporter is not regulated by any proteins, cofactors or substrates. Therefore mVenus does not only expand the spectrum of fluorescence proteins in the registry, it also is a good alternative for various genetic circuits.
Characteristica