Difference between revisions of "Part:BBa K1983018:Design"
(→‎Primers)
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VR: attaccgcctttgagtgagc <br>
 
VR: attaccgcctttgagtgagc <br>
  
−
Primers used for cloning a medium strength RBS: <br>
+
Primers used for cloning a low strength RBS: <br>
 
RBS-FW: TTGAATTCGCGGCCGCTTCTAGAGTTTACAGCTAGCTCAGTCCTAGGTATTATGCTAGCTAC <br>
 
RBS-FW: TTGAATTCGCGGCCGCTTCTAGAGTTTACAGCTAGCTCAGTCCTAGGTATTATGCTAGCTAC <br>
 
RBS-Low: GCATCTAGAAGTTCCACTCTTTCTCTAGTAGCTAGCATAATACCTAGGACTG <br>
 
RBS-Low: GCATCTAGAAGTTCCACTCTTTCTCTAGTAGCTAGCATAATACCTAGGACTG <br>
−
 
−
 
−
 
  
 
===Source===
 
===Source===

Revision as of 14:25, 21 October 2016


PheP under constitutive promoter, low strength RBS and terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 98
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Primers

Primers used for amplification of the fragment:
Uni-FW: GTAGAATTCGCGGCCGCTTCTAG
Uni-RV: GTAGACTGCAGCGGCCGCTACTAG

Primers used for colony PCR screening:

For pSB1C3:
VF2: tgccacctgacgtctaagaa
VR: attaccgcctttgagtgagc

Primers used for cloning a low strength RBS:
RBS-FW: TTGAATTCGCGGCCGCTTCTAGAGTTTACAGCTAGCTCAGTCCTAGGTATTATGCTAGCTAC
RBS-Low: GCATCTAGAAGTTCCACTCTTTCTCTAGTAGCTAGCATAATACCTAGGACTG

Source

BBa_K1983018

References