Revision as of 09:04, 21 October 2016

GS linker

Figure 1.GS Linker

Introduction

       The GS Linker(BBa_K1974030) that we provided is a 18 amino acid. It is useful for expressing functional fusion proteins. So, we wanted to use it to concent the spider toxin peptide and snowdrop lectin.

Modifying and Improving the Existed Biobrick

       Previous linker :
BBa_K416001 of 2010 iGEM10_NYU
GS Linker(BBa_K416001) is a 15 amino acid flexible peptide linker protein domain that is useful for creating functional fusion proteins. So we want to use it to concent the spider toxin peptide and snowdrop lectin.

       In the experiment, we hypothesized that an elongated linker would enhance the function of the fusion protein for the original linker is too short for the proper folding of the two domains. Futrhermore, we found no result in the previous history using the Flexible Peptide GS Linker(BBa_K416001) from 2010 NYU iGEM team. Therefore, we modified the sequence and added three more amino acids from into the linker. In the result, our improved GS Linker is completely proved by our experiments.

Result and Experiment

       In the experiment, the fusion protein with the elongated GS linker did show the enhanced repellent effect. Observed from the phenomenon, we did prove the enhanced function of the fusion proteins(see Result), so we speculate that the fusion protein has a better secondary structure than the original ones. As a result, we have submitted a basic part for the improved elongated GS linker.

Figure 2.GS linker. The DNA sequence length of GS linker is around 50-60 b.p. In this PCR experiment, the product’s size should be close to 300-350 b.p.

More application of this part

BBa_K1974033

Sequence and Features