Difference between revisions of "Part:BBa K1906004"

Revision as of 17:44, 20 October 2016

Elongation Factor Tu

This protein is well known as a translation elongation factor in bacterium Escherichia coli. It can also assemble with phage polymerase domain to form a RNA-dependent-RNA polymerase. It was suggested by XJTLU-CHINA 2016 iGEM team that over-expression of this protein together with EF-TS are essential for the production of beta subunit of Qbeta replicase.

Characterlization

It was quite surprising that plasmids that carry beta subunit genes don't lead to a heavy production of the protein upon the induction. However, this situation can be improved when EF-Ts and EF-Tu are co-expressed with beta subunit.

Figure 1 is the SDS-PAGE result comparison between cells that transformed with these three proteins and beta subunit alone.

Figure 1.SDS-PAGE result comparison between three-protein-expressing cells and beta subunit-expressing cells, wild-type cells were included as control group.

Sequence and Features