Difference between revisions of "Part:BBa I746200"

Revision as of 10:58, 8 September 2007

FepA L8T Mutant - Large Diffusion pore for E. coli outer membrane.

FepA is an outer membrane receptor protein for Escherichia coli involved in the uptake of the ferric enterobactin siderophore. This part is a deletion mutant of the wild type FepA, which has been shown to convert the protein into an open diffusion channel, similar to a porin. It allows larger molecules through than normally would be permitted. A total of 15 amino acid residues has been deleted from the wild type protein (see Fig. 2A in reference). These have been replaced by a beta turn to aid folding (NCET).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 238
Illegal AgeI site found at 604
Illegal AgeI site found at 1231
Illegal AgeI site found at 1749
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_I746200 short</partinfo>
-FepA is an outer membrane receptor protein for Escherichia coli involved in the uptake of the ferric enterobactin siderophore. This part is a deletion mutant of the wild type FepA, which has been shown to convert the protein into an open diffusion channel, similar to a porin. It allows larger molecules through than normally would be permitted.
+FepA is an outer membrane receptor protein for Escherichia coli involved in the uptake of the ferric enterobactin siderophore. This part is a deletion mutant of the wild type FepA, which has been shown to convert the protein into an open diffusion channel, similar to a porin. It allows larger molecules through than normally would be permitted. A total of 15 amino acid residues has been deleted from the wild type protein (see Fig. 2A in reference). These have been replaced by a beta turn to aid folding (NCET).
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