Difference between revisions of "Part:BBa K1906000"
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===Characterlization===
 
===Characterlization===
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It was
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[[File:20161019150339!SDS-TSTU-REP.jpg|200px|thumb|left|alt text]]
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[[File:SDS-TSTU-REP.jpg|200px|thumb|left|alt text]]
  
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 17:16, 20 October 2016


Elongation Factor Ts

This protein is well known as a translation elongation factor in bacterium Escherichia coli. It can also assemble with phage polymerase domain to form a RNA-dependent-RNA polymerase holoenzyme. It was suggested by XJTLU-CHINA 2016 iGEM team that overexpress of this protein together with EF-Tu are essential for the production of Qbeta replicase holoenzyme.

Characterlization

alt text
alt text

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 688
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 487