Difference between revisions of "Part:BBa K1906000"
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<partinfo>BBa_K1906000 short</partinfo> | <partinfo>BBa_K1906000 short</partinfo> | ||
− | This protein is well known as a translation elongation factor in bacterium Escherichia coli. It can also assemble with phage polymerase domain to form a RNA-dependent-RNA polymerase holoenzyme. It was suggested by XJTLU-CHINA 2016 iGEM team that overexpress of this protein together with EF- | + | This protein is well known as a translation elongation factor in bacterium Escherichia coli. It can also assemble with phage polymerase domain to form a RNA-dependent-RNA polymerase holoenzyme. It was suggested by XJTLU-CHINA 2016 iGEM team that overexpress of this protein together with EF-Tu are essential for the production of Qbeta replicase holoenzyme. |
===Characterlization=== | ===Characterlization=== |
Revision as of 16:55, 20 October 2016
Elongation Factor Ts
This protein is well known as a translation elongation factor in bacterium Escherichia coli. It can also assemble with phage polymerase domain to form a RNA-dependent-RNA polymerase holoenzyme. It was suggested by XJTLU-CHINA 2016 iGEM team that overexpress of this protein together with EF-Tu are essential for the production of Qbeta replicase holoenzyme.
Characterlization
It was
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 688
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 487