Difference between revisions of "Part:BBa K1980006"
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==References==
 
==References==
 
<p>(1) Danya J. Martell, Chandra P. Joshi, Ahmed Gaballa, Ace George Santiago, Tai-Yen Chen, Won Jung, John D. Helmann, and Peng Chen (2015) “Metalloregulator CueR biases RNA polymerase’s kinetic sampling of dead-end or open complex to repress or activate transcription” Proc Natl Acad Sci U S A. 2015 Nov 3; 112(44): 13467–13472</p>
 
<p>(1) Danya J. Martell, Chandra P. Joshi, Ahmed Gaballa, Ace George Santiago, Tai-Yen Chen, Won Jung, John D. Helmann, and Peng Chen (2015) “Metalloregulator CueR biases RNA polymerase’s kinetic sampling of dead-end or open complex to repress or activate transcription” Proc Natl Acad Sci U S A. 2015 Nov 3; 112(44): 13467–13472</p>
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<p style="text-align: right"><i>Author: Andreas Hadjicharalambous</i></p>

Revision as of 14:34, 23 October 2016


pCopA with divergent expressed CueR

Description

To facilitate making any protein under control of a copper-responsive promoter we designed this part. The Biobrick contains the constitutively expressed CueR (transcription factor). On the opposing direction we have the copper sensitive pCopA promoter but with nothing but an RBS inserted downstream of it. The RBS present means that any protein with the ATG biobrick prefix and universal suffix can be inserted with biobrick standard assembly with the correct RBS-ATG distance.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 438
    Illegal NheI site found at 461
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

The pCopA promoter was initially identified as the promoter of the copper exporting ATPase, CopA. The promoter contains inverted repeats of sequences called CueR boxes with the sequence: CCTTCCNNNNNNGGAAGG. This allows the binding of the DNA binding domains of the copper sensitive transcription factor CueR(1).

CueR is a MerR-type regulator with an interesting mechanism of action whereby it can behave as a net activator or a net repressor under different copper concentrations through interaction with RNA polymerase(1)


References

(1) Danya J. Martell, Chandra P. Joshi, Ahmed Gaballa, Ace George Santiago, Tai-Yen Chen, Won Jung, John D. Helmann, and Peng Chen (2015) “Metalloregulator CueR biases RNA polymerase’s kinetic sampling of dead-end or open complex to repress or activate transcription” Proc Natl Acad Sci U S A. 2015 Nov 3; 112(44): 13467–13472


Author: Andreas Hadjicharalambous