Difference between revisions of "Part:BBa K2070004:Experience"
 
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[[File:T--UT-Tokyo--BBa K2070005-1.png|thumb|700px| '''Measurement of ECF sigma35 and sigma30 promoter activity.''' Fluorescence/OD600 of a construct containing both an ECF sigma35 generating device and ECF sigma 35 inducible GFP generating device BBa_K2070005 was compared to that containing only either one of the devices. *** for P> 0.001 indicates an extremely significant difference between the induced and uninduced state. ]]
 
[[File:T--UT-Tokyo--BBa K2070005-1.png|thumb|700px| '''Measurement of ECF sigma35 and sigma30 promoter activity.''' Fluorescence/OD600 of a construct containing both an ECF sigma35 generating device and ECF sigma 35 inducible GFP generating device BBa_K2070005 was compared to that containing only either one of the devices. *** for P> 0.001 indicates an extremely significant difference between the induced and uninduced state. ]]
  
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[[File:T--UT-Tokyo--BBa K2070005-2.png|thumb|700px| '''Measurement of Activated Sigma30 Promoter Activity in Comparison to Promoter BBa_J23101. '''Both parts are ligated in plasmid pSB3K3. The part containing the constitutive sigma35 generator was double transformed into the cell for measurement. Data represents an average of 3 samples and error bars represent the standard  deviation.  
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We have also measured the strength of the activated promoter relative to BBa_J23101.
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[[File:T--UT-Tokyo--BBa K2070005-2.png|thumb|700px| '''Measurement of Activated Sigma30 Promoter Activity in Comparison to Promoter BBa_J23101. '''Both parts are ligated in plasmid pSB3K3. The part containing the constitutive sigma35 generator was double transformed into the cell for measurement. Data represents an average of 3 samples and error bars represent the standard  deviation. The promoter in its activated state is measured to have a RPU of 3.2 units.
 
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Latest revision as of 06:20, 25 October 2016


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BBa_K2070004 iGEM UT-Tokyo 2016

Our Experimental results show that the part work as expected, with the GFP expression higher in presence of ECF sigma35 expression.

Measurement of ECF sigma35 and sigma30 promoter activity. Fluorescence/OD600 of a construct containing both an ECF sigma35 generating device and ECF sigma 35 inducible GFP generating device BBa_K2070005 was compared to that containing only either one of the devices. *** for P> 0.001 indicates an extremely significant difference between the induced and uninduced state.

We have also measured the strength of the activated promoter relative to BBa_J23101.

Measurement of Activated Sigma30 Promoter Activity in Comparison to Promoter BBa_J23101. Both parts are ligated in plasmid pSB3K3. The part containing the constitutive sigma35 generator was double transformed into the cell for measurement. Data represents an average of 3 samples and error bars represent the standard deviation. The promoter in its activated state is measured to have a RPU of 3.2 units.
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