Difference between revisions of "Part:BBa F2620"

Revision as of 20:51, 19 October 2007

Input:	3OC₆HSL
Output:	PoPS

tetR R0040	LuxR I0462	lux pR R0062

Part Main Page Transfer Function Specificity Response time Stability Add Data

Description
A transcription factor [LuxR] (BBa_C0062) that is active in the presence of cell-cell signaling molecule 3OC₆HSL is controlled by a tetR regulatable operator (BBa_R0040). Device input is 3OC₆HSL. Device output is PoPS from a LuxR-regulated operator. If used in a cell containing TetR then a second input signal such as [http://openwetware.org/wiki/ATc aTc] can be used to produce a logical AND function. Get the pdf datasheet here.

Performance

Experiment¹	Characteristic¹	Value¹
Transfer Function	Input Swing	1E-9 - 1E-6 M 3OC₆HSL, exogenous
	Output Swing	0±1 - 503±1 GFP molecules cfu^-1 s^-1
	Switch Point	7E-9 ±1E-9 M 3OC₆HSL, exogenous
Response time:	Time from 0 - 50% output	9 min
	Time from 0 - 90% output	27 min
	Time from 100 - 50% output	# min
	Time from 100 - 5% output	# min
Specificity	Strong response to	3OC₆HSL, C₆HSL , C₇HSL, 3OC₈HSL, C₈HSL
Specificity	Weak response to	C₄HSL, C₁₀HSL, C₁₂HSL
Stability	Genetic Stability (Low/High Input)	>92/74 generations
Stability	Performance Stability (Low/High Input)	>92/74 generations
Demand	Translation Demand: (Low/High Input)	5040/141600 charged tRNA cfu^-1 s^-1
	Translation Demand: (Low/High Input)	336/9440 ribosomes sequestered cfu^-1
	Transcription Demand: (Low/High Input)	(#/#) nucleotides cfu^-1 s^-1
	Transcription Demand: (Low/High Input)	(#/#) RNAP sequestered cfu^-1
	Growth Rate (Low/High Input)	54/59 min doubling time

¹Measured by Ania Labno and Barry Canton 2006-2007

Compatibility
Chassis: Device has been shown to work in BBa_V1000,BBa_V1001,BBa_V1002
Plasmids: Device has been shown to work on pSB3K3 and pSB1A3
Devices: Device has been shown to work with BBa_E0430, BBa_E0434, BBa_E0240
Crosstalk with systems containing BBa_C0040.
Cell-Cell Signaling Systems: Crosstalk with devices using 3OC₆HSL, C6HSL, C7HSL, C8HSL, C10HSL.

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 <div style="padding: 00px; width: 680px; border: 0px solid #000000;">
 '''Description'''<br>
-A transcription factor [LuxR] (''<partinfo>C0062</partinfo>'') that is active in the presence of cell-cell signaling molecule [[3OC6HSL|3OC<sub>6</sub>HSL]] is controlled by a tetR regulatable operator (''<partinfo>BBa_R0040</partinfo>'').  ''Device input'' is [[3OC6HSL|3OC<sub>6</sub>HSL]].  ''Device output'' is [[PoPS]] from a LuxR-regulated operator.  If used in a cell containing TetR then a second input signal such as [http://openwetware.org/wiki/ATc aTc] can be used to produce a logical AND function.  Get the pdf datasheet  [[:Image:F2620submit.pdf|here]].
+A transcription factor [LuxR] (''<partinfo>C0062</partinfo>'') that is active in the presence of cell-cell signaling molecule [[3OC6HSL|3OC<sub>6</sub>HSL]] is controlled by a tetR regulatable operator (''<partinfo>BBa_R0040</partinfo>'').  ''Device input'' is [[3OC6HSL|3OC<sub>6</sub>HSL]].  ''Device output'' is [[PoPS]] from a LuxR-regulated operator.  If used in a cell containing TetR then a second input signal such as [http://openwetware.org/wiki/ATc aTc] can be used to produce a logical AND function.  Get the pdf datasheet  [[:IImage:F2620DataSheetV5.0.pdf|here]].