Difference between revisions of "Part:BBa K2070005"

 
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<partinfo>BBa_K2070005 short</partinfo>
 
<partinfo>BBa_K2070005 short</partinfo>
  
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This part was used to test the functionality of ECF sigma35 and its cognate promoter.  
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This part, consisting of a constitutive ECF sigma35 generator [https://parts.igem.org/Part:BBa_K2070013 BBa_K2070013] and ECF sigma35 inducible GFP reporter [https://parts.igem.org/Part:BBa_K2070004 BBa_K2070004] and was used to test the functionality of ECF sigma35 and its cognate promoter.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 11:57, 20 October 2016


Constitutive ecf35 generator with ecf35 inducible GFP reporter

This part, consisting of a constitutive ECF sigma35 generator BBa_K2070013 and ECF sigma35 inducible GFP reporter BBa_K2070004 and was used to test the functionality of ECF sigma35 and its cognate promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 790
    Illegal BsaI.rc site found at 1543