Difference between revisions of "Part:BBa K2120409"

Revision as of 08:41, 20 October 2016

B0015+cI+B0034+J23106+pR+B0034+mazF+B0015

This is one of our application circuit.

This circuit consists of two parts:the inhibitor device and killer device ,which have already been made into basic parts of biobricks.

We choose three different promoters (BBa_J23109,BBa_J23116,BBa_J23106) whose reported strengths are respectively low, medium and strong from constitutive promoter family to express the inhibitor tetR and cI ,and then we construct three plasmids with different copy number containing this circuit, in order to find out the proper promoter which meets the threshold that trigger the killer system in E.coli. According to our results about the lethal effect of killer device , MazF ,so we replace the killer gene with mRFP reporter gene in order to figure out the regulation relationship between two devices.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 941
Illegal NheI site found at 964
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 6: / Line 6: @@
 This is one of our application circuit.
-Our application circuit circuit consists of two parts:the inhibitor device and killer device ,which have already been made into basic parts of biobricks.
+This circuit consists of two parts:the inhibitor device and killer device ,which have already been made into basic parts of biobricks.
-We choose four different promoters whose reported strengths are respectively low, medium,strong and the strongest from constitutive promoter family to express the inhibitor tetR and cI ,and then we construct three plasmid with different copy number containing this circuit, in order to find out the proper promoter which meets the threshold that trigger the killer system.
+We choose three different promoters (BBa_J23109,BBa_J23116,BBa_J23106) whose reported strengths are respectively low, medium and strong from constitutive promoter family to express the inhibitor tetR and cI ,and then we construct three plasmids with different copy number containing this circuit, in order to find out the proper promoter which meets the threshold that trigger the killer system in E.coli. According to our results about the lethal effect of killer device , [https://parts.igem.org/wiki/index.php?title=Part:BBa_K212008 MazF] ,so we replace the killer gene with mRFP reporter gene in order to figure out the regulation relationship between two devices.
-Go our [https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2016&group=BIT-China Parts Registry] to learn more.
 <!-- Add more about the biology of this part here