Difference between revisions of "Part:BBa K2120401"
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<partinfo>BBa_K2120401 short</partinfo>
 
<partinfo>BBa_K2120401 short</partinfo>
  
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This is one of our application circuit.
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'''<Description>'''
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<br>We select the strong constitutive promoter J23119 and strong RBS B0034 to express inhibitor of pTet, tetR. And we ligate induced promoter pTet following the inhibitor. In order to decrease impact between constitutive promoter and induced promoter, we reserve the constitutive promoter’s direction and the transcription of it will not impact the downstream killer. Then killer, ''mazF'', which locates at downstream of induced promoter will be regulated by concentration of inhibitor connecting plasmid copy numbers in a suitable range. Then this device can kill the cell with little plasmids and maintain a high expression level of function gene in industrial production.
  
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Our application circuit circuit consists of two parts:the inhibitor device and killer device ,which have already been made into basic parts of biobricks.
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<br>Go our [https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2016&group=BIT-China Parts Registry] to learn more.
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We choose four different promoters whose reported strengths are respectively low, medium,strong and the strongest from constitutive promoter family to express the inhibitor tetR and cI ,and then we construct three plasmid with different copy number containing this circuit, in order to find out the proper promoter which meets the threshold that trigger the killer system.
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Go our [https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2016&group=BIT-China Parts Registry] to learn more.
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Revision as of 06:11, 23 October 2016


B0015+tetR+B0034+J23119+pTet+B0032+mazF+B0015

<Description>
We select the strong constitutive promoter J23119 and strong RBS B0034 to express inhibitor of pTet, tetR. And we ligate induced promoter pTet following the inhibitor. In order to decrease impact between constitutive promoter and induced promoter, we reserve the constitutive promoter’s direction and the transcription of it will not impact the downstream killer. Then killer, mazF, which locates at downstream of induced promoter will be regulated by concentration of inhibitor connecting plasmid copy numbers in a suitable range. Then this device can kill the cell with little plasmids and maintain a high expression level of function gene in industrial production.


Go our Parts Registry to learn more.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 851
    Illegal NheI site found at 874
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]