Difference between revisions of "Part:BBa K2150123"
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This basic part is a CDS for toxin 5694 in our project. | This basic part is a CDS for toxin 5694 in our project. | ||
In our final construct, this gene is controlled by aTc, and we characterized this part by measuring the growth curve of E. coli induced by graded aTc. | In our final construct, this gene is controlled by aTc, and we characterized this part by measuring the growth curve of E. coli induced by graded aTc. | ||
| − | During the experiment, the gene does not have a RBS upstream to it, because evening choosing the weakest RBS ( | + | During the experiment, the gene does not have a RBS upstream to it, because evening choosing the weakest RBS (BBa_B0033) make it unable for us to get the right colony. |
This also provides an opportunity for future teams to characterize or improve or use this part. | This also provides an opportunity for future teams to characterize or improve or use this part. | ||
Latest revision as of 03:35, 20 October 2016
5694
This basic part is a CDS for toxin 5694 in our project. In our final construct, this gene is controlled by aTc, and we characterized this part by measuring the growth curve of E. coli induced by graded aTc. During the experiment, the gene does not have a RBS upstream to it, because evening choosing the weakest RBS (BBa_B0033) make it unable for us to get the right colony. This also provides an opportunity for future teams to characterize or improve or use this part.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]