Difference between revisions of "Part:BBa K2150115"
 
Line 12: Line 12:
 
We also characterized its corresponding toxin, ParE, see:  
 
We also characterized its corresponding toxin, ParE, see:  
 
[https://parts.igem.org/Part:BBa_K2150111 BBa_K2150111]
 
[https://parts.igem.org/Part:BBa_K2150111 BBa_K2150111]
 +
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 02:55, 20 October 2016


6249 antitoxin tet

This antitoxin belongs to ParD family. It can effectively neutralize the toxin ParE. We characterized this by measuring the growth curve co-expressing toxin and antitoxin.

This protein is induced by aTc. When studying the interaction between antitoxin and toxin, antitoxin is induced by IPTG.

See: BBa_K2150117

We also characterized its corresponding toxin, ParE, see: BBa_K2150111

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 357
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]