Difference between revisions of "Part:BBa K1952011"
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<partinfo>BBa_K1952011 short</partinfo> | <partinfo>BBa_K1952011 short</partinfo> | ||
− | Hydrazine Synthase subunit beta (Kust-2860) with strong RBS under lactose-inducible promoter, followed | + | Hydrazine Synthase subunit beta (Kust-2860) with strong RBS under lactose-inducible promoter, followed by LacZ alpha subunit reporter gene. |
− | + | Used to verify proper expression of Hydrazine Synthase beta subunit in E. coli (demonstrate expression by blue color of bacteria in the presence of IPTG and X-gal.) | |
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Latest revision as of 15:54, 29 October 2016
Hydrazine Synthase subunit beta (Kust-2860) with LacZ reporter
Hydrazine Synthase subunit beta (Kust-2860) with strong RBS under lactose-inducible promoter, followed by LacZ alpha subunit reporter gene.
Used to verify proper expression of Hydrazine Synthase beta subunit in E. coli (demonstrate expression by blue color of bacteria in the presence of IPTG and X-gal.)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 966
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]