Difference between revisions of "Part:BBa K1952011"

 
 
Line 3: Line 3:
 
<partinfo>BBa_K1952011 short</partinfo>
 
<partinfo>BBa_K1952011 short</partinfo>
  
Hydrazine Synthase subunit beta (Kust-2860) with strong RBS under lactose-inducible promoter, followed  
+
Hydrazine Synthase subunit beta (Kust-2860) with strong RBS under lactose-inducible promoter, followed by LacZ alpha subunit reporter gene.
  
by LacZ alpha subunit reporter gene.
+
Used to verify proper expression of Hydrazine Synthase beta subunit in E. coli (demonstrate expression by blue color of bacteria in the presence of IPTG and X-gal.)
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 15:54, 29 October 2016


Hydrazine Synthase subunit beta (Kust-2860) with LacZ reporter

Hydrazine Synthase subunit beta (Kust-2860) with strong RBS under lactose-inducible promoter, followed by LacZ alpha subunit reporter gene.

Used to verify proper expression of Hydrazine Synthase beta subunit in E. coli (demonstrate expression by blue color of bacteria in the presence of IPTG and X-gal.)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 966
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]