Difference between revisions of "Part:BBa K1909009"
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<partinfo>BBa_K1909009 short</partinfo> | <partinfo>BBa_K1909009 short</partinfo> | ||
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BBa_K1909009 is a construct of medium [0.33] Anderson promoter BBa_K608007, a weak [0.6] ribosome binding site and the receptor mTaz, part BBa_K1909002, capable of detecting meso-2,6-Diaminopimelic acid (mDAP). | BBa_K1909009 is a construct of medium [0.33] Anderson promoter BBa_K608007, a weak [0.6] ribosome binding site and the receptor mTaz, part BBa_K1909002, capable of detecting meso-2,6-Diaminopimelic acid (mDAP). | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
The receptor mTaz is based on the chemotaxis receptor Tar, part BBa_C0082, capable of sensing aspartate and is in combination with the cytosolic domain of osmosensor kinase EnvZ. The combination of BBa_K608007 and BBa_C0082 is part BBa_K1909012. | The receptor mTaz is based on the chemotaxis receptor Tar, part BBa_C0082, capable of sensing aspartate and is in combination with the cytosolic domain of osmosensor kinase EnvZ. The combination of BBa_K608007 and BBa_C0082 is part BBa_K1909012. |
Latest revision as of 22:45, 19 October 2016
Weak Anderson / mTaz
BBa_K1909009 is a construct of medium [0.33] Anderson promoter BBa_K608007, a weak [0.6] ribosome binding site and the receptor mTaz, part BBa_K1909002, capable of detecting meso-2,6-Diaminopimelic acid (mDAP).
Usage and Biology
The receptor mTaz is based on the chemotaxis receptor Tar, part BBa_C0082, capable of sensing aspartate and is in combination with the cytosolic domain of osmosensor kinase EnvZ. The combination of BBa_K608007 and BBa_C0082 is part BBa_K1909012.
Functional Parameters
This combination has the lowest expression of the three combinations BBa_K1909007, BBa_K1909008 and BBa_K1909009. Testing the combination showed that the cells grew very, very slow (for miniprep culture more than 48 hours, for colonies on LB-agar plates more than 72 hours).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 174