Difference between revisions of "Part:BBa K2150015"

Latest revision as of 00:55, 20 October 2016

pTet TetX-GFP(fusion protein)

This part is tetX-GFP with pTet promoter.

Usage and Biology

We use this part to create an antibiotic scavenger in the presence of tetR. The performance of this part was compared with BBa_K2150027 when tetR existed in the cell. The data showed BBa_K2150027 had a better capacity of degrading tetracycline than this part(see BBa_K21500028).

Characterization

This part showed a lower fluoresence intensity than BBa_2150014 at the same growth stage of E.coli (see BBa_K2150013).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 642
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1939

@@ Line 6: / Line 6: @@
 ===Usage and Biology===
-We use this part to create an antibiotic scavenger in the presence of tetR. The performance of this part is compared with BBa_K2150027 when tetR exists in the cell. The data showes BBa_K2150027 has a better capacity of degrading tetracycline than this part(see [https://parts.igem.org/Part:BBa_K2150028 BBa_K21500028]).
+We use this part to create an antibiotic scavenger in the presence of tetR. The performance of this part was compared with BBa_K2150027 when tetR existed in the cell. The data showed BBa_K2150027 had a better capacity of degrading tetracycline than this part(see [https://parts.igem.org/Part:BBa_K2150028 BBa_K21500028]).
 ===Characterization===
-This part shows a lower fluoresence intensity than BBa_2150014 at the same growth stage of E.coli (see [https://parts.igem.org/Part:BBa_K2150013 BBa_K2150013]).
+This part showed a lower fluoresence intensity than BBa_2150014 at the same growth stage of E.coli (see [https://parts.igem.org/Part:BBa_K2150013 BBa_K2150013]).
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