Difference between revisions of "Part:BBa K1909013"
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<partinfo>BBa_K1909013 short</partinfo> | <partinfo>BBa_K1909013 short</partinfo> | ||
Promoter (OmpR, positive) with GFP | Promoter (OmpR, positive) with GFP | ||
− | The part BBa_K1909013 consists of the green fluorescent protein GFP and the positively regulated OmpR-controlled promoter (BBa_R0082). The promoter OmpR is taken from the upstream region of ompC. Phosphorylated OmpR binds to the three operator sites and activates transcription | + | |
− | + | The part BBa_K1909013 consists of the green fluorescent protein GFP and the positively regulated OmpR-controlled promoter (BBa_R0082). The promoter OmpR is taken from the upstream region of ompC. Phosphorylated OmpR binds to the three operator sites and activates transcription. | |
− | + | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
+ | In nature, the promoter OmpR is upstream of the ompC porin gene. The regulation of ompC is determined by the EnvZ-OmpR osmosensing machinery. EnvZ phosphorylates OmpR to OmpR-P. At high osmolarity, EnvZ is more active, creating more OmpR-P. OmpR-P then binds to the low-affinity OmpR operator sites upstream of ompC. Via FACS analysis it was shown in EnvZ deficient cells (JW3367-3), containing the construct BBa_K1909013 ,that the expression of GFP was significantly reduced in comparison to EnvZ containing cells (DH5α). | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K1909013 SequenceAndFeatures</partinfo> | <partinfo>BBa_K1909013 SequenceAndFeatures</partinfo> |
Revision as of 21:04, 19 October 2016
Omp Promoter / GFP
Promoter (OmpR, positive) with GFP
The part BBa_K1909013 consists of the green fluorescent protein GFP and the positively regulated OmpR-controlled promoter (BBa_R0082). The promoter OmpR is taken from the upstream region of ompC. Phosphorylated OmpR binds to the three operator sites and activates transcription.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 779