Difference between revisions of "Part:BBa I766557:Experience"

 
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<I>Username</I>
 
<I>Username</I>
 
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UCBerkeley2012 Team: To assay their potential use in our project, we characterized the strength of several registry yeast promoters. We wanted to compare their consistency of expression and relative fluorescence against our lab's strongest promoter, pTDH3. The promoters we used in our assay: pSTE5 (weak), pCYC1 (medium),pADH1 (strong), and  pTDH3 (strongest). <br>
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''Experimental Design:''' We designed our promoters to express a fluorescent protein so that we could quickly measure bulk fluorescence via TECAN. To roughly test if downstream sequence affects expression, we cloned the promoter in front of two different fluorescent proteins, yellow fluorescent Venus and red fluorescent mKate. The device was cloned on a backbone with Leu2 marker and Cen6 origin of replication.
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[[File:https://static.igem.org/mediawiki/2012/0/0f/IGEM_promoter_characterization.png|thumb|none|200px| Promoter Characterization graph ]]
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Revision as of 20:30, 2 October 2012

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_I766557

User Reviews

Status: 500 Content-type: text/html

Software error:

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Status: 500 Content-type: text/html

Software error:

Not enough arguments for Range::range at /websites/parts.igem.org/cgi/lib/Range.pm line 250, near "range;"
Unmatched right curly bracket at /websites/parts.igem.org/cgi/lib/Range.pm line 251, at end of line
syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 251, near "}"
Can't use global @_ in "my" at /websites/parts.igem.org/cgi/lib/Range.pm line 261, near "= @_"
syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 282, near "}"
Can't use global @_ in "my" at /websites/parts.igem.org/cgi/lib/Range.pm line 286, near "= @_"
Global symbol "$course" requires explicit package name at /websites/parts.igem.org/cgi/lib/Range.pm line 288.
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Compilation failed in require at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.

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