Difference between revisions of "Part:BBa I766557"
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<partinfo>BBa_I766557 short</partinfo> | <partinfo>BBa_I766557 short</partinfo> | ||
− | + | Low expression, constitutive promoter in yeast<br> | |
+ | Induced by α-factor-pheromone (Printen & Sprague, 1994) <br> | ||
+ | Ste5 scaffold protein; ensures assembly of the protein kinases of the pheromone-activated MAPK cascade into an active complex during mating (Printen & Sprague, 1994) | ||
− | |||
===Usage and Biology=== | ===Usage and Biology=== | ||
− | + | The comparative study on the abundance of the Ste5 scaffold protein depending on the promoter was conducted by Chapman & Asthagiri in 2009 ('''Figure 1'''). As can be seen from Figure 1, STE5 promoter is a very weak promoter, which ensures a lower expression level in comparison even with CYC promoter. | |
+ | [[File:1(2).jpg|thumb|center|500px|Figure 1. The relative abundance of STE5-MYC-tagged protein expressed from inducible STE5 (induced by α-factor) and constitutive CYC, ADH, TEF, and GPD promoters. The error bars represent SEM (n=3) (Chapman & Asthagiri, 2009). | ||
+ | ]] | ||
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+ | <br> | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_I766557 SequenceAndFeatures</partinfo> | <partinfo>BBa_I766557 SequenceAndFeatures</partinfo> | ||
− | + | '''References''' <br> | |
+ | Chapman, S. A., & Asthagiri, A. R. (2009). Quantitative effect of scaffold abundance on signal propagation. Molecular Systems Biology, 5(1), 313. https://doi.org/10.1038/msb.2009.73 <br> | ||
+ | Printen, J. A., & Sprague, G. F. (1994). Protein-protein interactions in the yeast pheromone response pathway: Ste5p interacts with all members of the MAP kinase cascade. Genetics, 138(3), 609–619. | ||
<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display | ||
===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_I766557 parameters</partinfo> | <partinfo>BBa_I766557 parameters</partinfo> | ||
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Revision as of 04:44, 24 October 2020
pSte5 (Weak) Promoter
Low expression, constitutive promoter in yeast
Induced by α-factor-pheromone (Printen & Sprague, 1994)
Ste5 scaffold protein; ensures assembly of the protein kinases of the pheromone-activated MAPK cascade into an active complex during mating (Printen & Sprague, 1994)
Usage and Biology
The comparative study on the abundance of the Ste5 scaffold protein depending on the promoter was conducted by Chapman & Asthagiri in 2009 (Figure 1). As can be seen from Figure 1, STE5 promoter is a very weak promoter, which ensures a lower expression level in comparison even with CYC promoter.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 93
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 93
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 93
- 1000COMPATIBLE WITH RFC[1000]
References
Chapman, S. A., & Asthagiri, A. R. (2009). Quantitative effect of scaffold abundance on signal propagation. Molecular Systems Biology, 5(1), 313. https://doi.org/10.1038/msb.2009.73
Printen, J. A., & Sprague, G. F. (1994). Protein-protein interactions in the yeast pheromone response pathway: Ste5p interacts with all members of the MAP kinase cascade. Genetics, 138(3), 609–619.