Difference between revisions of "Part:BBa K1021003"
Chilam Poon (Talk | contribs) |
Chilam Poon (Talk | contribs) |
||
Line 34: | Line 34: | ||
− | + | We did the glufosinate ammonium inhibition test, the data is showed below. | |
The initial radius were all 4mm. The mycelium length was measured on day 4, 5 and 6 after starting ''M.anisopliae'' conidia cultivation on solid media. | The initial radius were all 4mm. The mycelium length was measured on day 4, 5 and 6 after starting ''M.anisopliae'' conidia cultivation on solid media. |
Revision as of 18:51, 19 October 2016
bar (bialaphos resistance)
The bialaphos resistance (bar) gene confers resistance to bialaphos and phosphinothricin.
Usage and Biology
The common fungus transformation system of entomopathogen Metarhizium anisopliae was based on resistance to the herbicide glufosinate ammonium (phosphinothricin), conferred by the bar gene.
Team NYMU 2016 characterized the bar marker gene, a twin of this part, using the bar expression cassette on vector pBARGPE1 with our chassis organism M.anisopliae.
- The transformants carrying the bar gene can grow on the PDA containing glufosinate ammonium.
We did the glufosinate ammonium inhibition test, the data is showed below.
The initial radius were all 4mm. The mycelium length was measured on day 4, 5 and 6 after starting M.anisopliae conidia cultivation on solid media.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 545
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 54
Illegal NgoMIV site found at 205
Illegal NgoMIV site found at 451 - 1000COMPATIBLE WITH RFC[1000]