Difference between revisions of "Part:BBa K1943004:Experience"

(Applications of BBa_K1943004)
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===Applications of BBa_K1943004===
 
===Applications of BBa_K1943004===
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After we constructed this part, we transformed it into BL21 to test the expression efficiency. Meanwhile, we did a comparison with another part whose promoter+RBS combination differ.
  
 
===Expression test of BBa_K1943004===
 
===Expression test of BBa_K1943004===

Revision as of 18:15, 19 October 2016


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1943004

After we constructed this part, we transformed it into BL21 to test the expression efficiency. Meanwhile, we did a comparison with another part whose promoter+RBS combination differ.

Expression test of BBa_K1943004

We spread the plate for DH5α at 1:20am on 14th, Oct. The plate contains chloramphenicol. Following photo were taken every several hours after placing the plates into a 37℃ incubator. K1943004 is in the up-left corner in each photo.

At 12:24 (14th,Oct.)

6 7@1224.jpeg

At 14:20 (14th,Oct.)

6 7@1420.jpeg

At 18:30 (14th,Oct.)

6 7@1830.jpeg

At 20:21 (14th,Oct.)

6 7@2021.jpeg

At 22:40 (14th,Oct.)

6 7@2240.jpeg

At 8:50 (15th,Oct.)

6 7@850.jpeg

At 14:28 (15th,Oct.)

6 7@1428.jpeg

User Reviews

UNIQ1fc1c5626b0ded7e-partinfo-00000000-QINU UNIQ1fc1c5626b0ded7e-partinfo-00000001-QINU