Difference between revisions of "Part:BBa J04450"

Revision as of 19:18, 19 October 2016

RFP Coding Device

LacI R0010	B0034	mRFP1 E1010	B0015

The colonies are clearly red in color under natural light after about 18 hours. Smaller colonies are visibly red under UV. The RFP part does not contain a degradation tag and the RBS is strong.

LacI sensitive
CAP sensitive

This part is commonly used, but can fail if the system contains LacI or CAP protein.
(--Meagan 15:39, 23 July 2009 (UTC))

[http://2012.igem.org/Team:TU_Munich Team TU_Munich 2012] improved this part by making it compatible to RFC10 and RFC25 (see: BBa_K801100)
(--VolkerMorath 15:02, 21 October 2012 (UTC))

[http://2013.igem.org/Team:NRP-UEA-Norwich Team NRP-UEA 2013] improved this part by adding a NdeI restriction site before the RFP gene. (see: BBa_K1041000)
(--holusac 20:46, 14 August 2013 (UTC))

[http://2015.igem.org/Team:Warwick Team Warwick 2015] improved this part by analysing the effect of copy number on gene expression.
(--Lcarroll 20:48, 25 September 2015 (UTC))

[http://2016.igem.org/Team:Leiden Team Leiden 2016] contributed to the characterisation of this part by showing equal functionality in simulated microgravity (0g) as in the normal gravity of the Earth.
(--Valentijn 19:38, 19 October 2016 (UTC))

Pictures

BBa_J04450 visualized under non-UV lightbox
BBa_J04450 visualized under 254nm wavelength UV lightbox
BBa_J04450 Colonies
==Usage as a cloning tool==
==Usage in Chromobacterium Violaceum==

@@ Line 31: / Line 31: @@
 Image:J04450 - UV 254nm.jpg|BBa_J04450 visualized under 254nm wavelength UV lightbox
 Image:J04500-colonies.jpg|BBa_J04450 Colonies
+image:
-</gallery>
 ==Usage as a cloning tool==