Difference between revisions of "Part:BBa I20248"
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Device is based on I2055 and contains a partial biobrick site (EcoR1, Not1, Xba1) between R0040 and GFP. To insert a BB promoter for testing, digest the promoter EcoR1 and Spe1 and the kit EcoR1 and Xba1. The result will form a full BB prefix upstream of the RBS and a mixed site downstream. The full BB prefix has a larger spacing than would occur in actual construction. | Device is based on I2055 and contains a partial biobrick site (EcoR1, Not1, Xba1) between R0040 and GFP. To insert a BB promoter for testing, digest the promoter EcoR1 and Spe1 and the kit EcoR1 and Xba1. The result will form a full BB prefix upstream of the RBS and a mixed site downstream. The full BB prefix has a larger spacing than would occur in actual construction. | ||
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+ | Note: sequence features are wrong | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Latest revision as of 13:30, 16 August 2007
Device used to characterize Ribosome Binding Sites through measurement of GFP expression
Device is based on I2055 and contains a partial biobrick site (EcoR1, Not1, Xba1) between R0040 and GFP. To insert a BB promoter for testing, digest the promoter EcoR1 and Spe1 and the kit EcoR1 and Xba1. The result will form a full BB prefix upstream of the RBS and a mixed site downstream. The full BB prefix has a larger spacing than would occur in actual construction.
Note: sequence features are wrong
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 61
Illegal XbaI site found at 75 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 61
Illegal NotI site found at 67 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 61
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 61
Illegal XbaI site found at 75 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 61
Illegal XbaI site found at 75 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 723