Difference between revisions of "Part:BBa I20245"
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Device is based on E0240 and contains a partial biobrick site (EcoR1, Not1, Xba1) upstream of the RBS. To insert a BB promoter for testing, digest the promoter EcoR1 and Spe1, and the result will form a mixed site. This design best simulates spacing in actual construction however, parts cannot be removed after testing. | Device is based on E0240 and contains a partial biobrick site (EcoR1, Not1, Xba1) upstream of the RBS. To insert a BB promoter for testing, digest the promoter EcoR1 and Spe1, and the result will form a mixed site. This design best simulates spacing in actual construction however, parts cannot be removed after testing. | ||
+ | |||
+ | Note: Sequence Features are wrong | ||
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Latest revision as of 13:25, 16 August 2007
A device used to characterize promoters throught measurement of GFP expression
Device is based on E0240 and contains a partial biobrick site (EcoR1, Not1, Xba1) upstream of the RBS. To insert a BB promoter for testing, digest the promoter EcoR1 and Spe1, and the result will form a mixed site. This design best simulates spacing in actual construction however, parts cannot be removed after testing.
Note: Sequence Features are wrong
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1
Illegal XbaI site found at 15 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal NotI site found at 7 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1
Illegal XbaI site found at 15 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1
Illegal XbaI site found at 15 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 684