Difference between revisions of "Part:BBa I20246"
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Device is based on I2057 and contains a partial biobrick site (EcoR1, Not1, Spe1) upstream of the RBS. To insert a BB promoter for testing, digest both the promoter and measurement device with EcoR1 and Spe1. Parts can be removed after testing, which facilitates the screening of libraries. | Device is based on I2057 and contains a partial biobrick site (EcoR1, Not1, Spe1) upstream of the RBS. To insert a BB promoter for testing, digest both the promoter and measurement device with EcoR1 and Spe1. Parts can be removed after testing, which facilitates the screening of libraries. | ||
− | + | Note: Sequence features are wrong | |
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Latest revision as of 13:26, 16 August 2007
A device used to characterize promoters throught measurement of RFP expression
Device is based on I2057 and contains a partial biobrick site (EcoR1, Not1, Spe1) upstream of the RBS. To insert a BB promoter for testing, digest both the promoter and measurement device with EcoR1 and Spe1. Parts can be removed after testing, which facilitates the screening of libraries.
Note: Sequence features are wrong
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1
Illegal SpeI site found at 15 - 12INCOMPATIBLE WITH RFC[12]Illegal prefix found in sequence at 1
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1
Illegal SpeI site found at 15 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1
Illegal SpeI site found at 15
Illegal AgeI site found at 595
Illegal AgeI site found at 707 - 1000COMPATIBLE WITH RFC[1000]