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<br><br>The biosynthetic locus of mutacin III was designed by our team in a form allowing for high-yield and fine-tuned expression of the peptide (Fig. 1). We placed a strong T7 promoter upstream of mutA to obtain high levels of the propeptide, a repressible pTet promoter upstream of the mutBCDP co-transcription unit and an inducible araBAD promoter for the mutT gene, coding for the ATP-binding-cassette-like transporter of mutacin III. All illegal restriction sites were removed from the endogenous sequences by silent mutagenesis.
 
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<p> <center> Fig. 1. Simplified diagram of the mutacin III biosynthetic locus designed by UCL iGEM 2016. </center> </p>
 
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<partinfo>BBa_K1954004 SequenceAndFeatures</partinfo>
 
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<partinfo>BBa_K729004 short</partinfo>
 
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<br><br>The biosynthetic locus of mutacin III was designed by our team in a form allowing for high-yield and fine-tuned expression of the peptide (Fig. 1). We placed a strong T7 promoter upstream of mutA to obtain high levels of the propeptide, a repressible pTet promoter upstream of the mutBCDP co-transcription unit and an inducible araBAD promoter for the mutT gene, coding for the ATP-binding-cassette-like transporter of mutacin III. All illegal restriction sites were removed from the endogenous sequences by silent mutagenesis.
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<partinfo>BBa_K729004 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K729004 SequenceAndFeatures</partinfo>
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===Design Notes===
 
===Design Notes===
 
All restriction sites were removed by silent mutagenesis.
 
All restriction sites were removed by silent mutagenesis.
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<center> <img src ="https://static.igem.org/mediawiki/2016/0/07/T--UCL--bacteriocindevice.png" width="50%" height="50%" </center></html>
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<p> <center> Fig. 1. Simplified diagram of the mutacin III biosynthetic locus designed by UCL iGEM 2016. </center> </p>
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Revision as of 16:33, 19 October 2016

Nuclease from Staphylococcus aureus



The biosynthetic locus of mutacin III was designed by our team in a form allowing for high-yield and fine-tuned expression of the peptide (Fig. 1). We placed a strong T7 promoter upstream of mutA to obtain high levels of the propeptide, a repressible pTet promoter upstream of the mutBCDP co-transcription unit and an inducible araBAD promoter for the mutT gene, coding for the ATP-binding-cassette-like transporter of mutacin III. All illegal restriction sites were removed from the endogenous sequences by silent mutagenesis.



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

All restriction sites were removed by silent mutagenesis.


Fig. 1. Simplified diagram of the mutacin III biosynthetic locus designed by UCL iGEM 2016.



Source

Our device was based on the sequence of AF154675.1

References