Difference between revisions of "Part:BBa K2036009"

Revision as of 16:28, 19 October 2016

pRM-GFP-LVAssrAtag

pRM-GFP-LVAtag, the circuit can function as a control group of Cro and pRM interaction characterization with test group: Cro-TT-pRM-RBS-GFP-LVAssrAtag (BBa_K20360010).

Fig1:Cro&pRM interaction characterization circuit

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 699

Protein&promoter

--Cro and pRM

Fig2: We characterized cro and pRM inhibition by the same method as CI and pR’s. From line chart and fluorescence detection, we can see that the test group contains cro expressed less GFP protein than control group over time. It proves that cro can effectively bind pRM to block its downstream gene’s transcription.

@@ Line 22: / Line 22: @@
 <p>--Cro and pRM</p>
 <br>
-[[File:T--HUST-China--CI-pR_inhibition.png|800px|thumb|center|Fig1: We characterized cro and pRM inhibition by the same method as CI and pR’s. From line chart and fluorescence detection, we can see that the test group contains cro expressed less GFP protein than control group over time. It proves that cro can effectively bind pRM to block its downstream gene’s transcription.]]
+[[File:T--HUST-China--CI-pR_inhibition.png|800px|thumb|center|Fig2: We characterized cro and pRM inhibition by the same method as CI and pR’s. From line chart and fluorescence detection, we can see that the test group contains cro expressed less GFP protein than control group over time. It proves that cro can effectively bind pRM to block its downstream gene’s transcription.]]
 <br>