Difference between revisions of "Part:BBa K1991005"
Line 1: | Line 1: | ||
− | [[ | + | __NOTOC__ |
+ | <partinfo>BBa_K1991005 short</partinfo> | ||
+ | |||
+ | AOX1 gene ([https://parts.igem.org/Part:BBa_K1991000 BBa_K1991000])was fused with Lpp-OmpA ([https://parts.igem.org/Part:BBa_K1991004 BBa_K1991004]) on pSB1C3. Lpp-OmpA can direct AOX1 protein on E. coli cell surface. | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <!-- Add more about the biology of this part here | ||
+ | ===Usage and Biology=== | ||
+ | |||
+ | <!-- --> | ||
+ | <span class='h3bb'>Sequence and Features</span> | ||
+ | <partinfo>BBa_K1991005 SequenceAndFeatures</partinfo> | ||
+ | |||
+ | |||
+ | <!-- Uncomment this to enable Functional Parameter display | ||
+ | ===Functional Parameters=== | ||
+ | <partinfo>BBa_K1991005 parameters</partinfo> | ||
+ | <!-- --> |
Revision as of 18:06, 19 October 2016
LO-AOX1-His (Lpp-OmpA-Alcohol Oxidase)
AOX1 gene (BBa_K1991000)was fused with Lpp-OmpA (BBa_K1991004) on pSB1C3. Lpp-OmpA can direct AOX1 protein on E. coli cell surface.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 2437
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 436
Illegal XhoI site found at 2446 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 391
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2128