Difference between revisions of "Part:BBa K1906003"

Revision as of 09:08, 20 October 2016

Intron encoded protein LtrA

LtrA is a protein that encoded by Lactococcus lactis LtrB intron. LtrA is a multifunctional protein that promotes group II intron splicing and mobility.

It has three activities: reverse transcriptase (RT) for intron duplication, maturase to promote splicing, and DNA endonuclease for site-specific cleavage of recipient alleles. The intron-encoded protein promotes splicing by facilitating the formation of the catalytically active structure of the intron RNA. After splicing, the protein remains bound to the excised intron lariat RNA, forming ribonucleoprotein particles, and cleaving the antisense strand of the recipient DNA in the 3' exon. After DNA cleavage, retrohoming occurs by a target DNA-primed reverse transcription of the intron RNA that had reverse spliced into the sense strand of the recipient DNA. It also contributes to the recognition of the DNA target site and acts as a repressor of its own translation.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1093
Illegal BamHI site found at 1469
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 369
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 291

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K1906003 short</partinfo>
-LtrA protein encoded by intron.
+LtrA is a protein that encoded by <i>Lactococcus lactis</i> LtrB intron. LtrA is a multifunctional protein that promotes group II intron splicing and mobility.
+It has three activities: reverse transcriptase (RT) for intron duplication, maturase to promote splicing, and DNA endonuclease for site-specific cleavage of recipient alleles. The intron-encoded protein promotes splicing by facilitating the formation of the catalytically active structure of the intron RNA. After splicing, the protein remains bound to the excised intron lariat RNA, forming ribonucleoprotein particles, and cleaving the antisense strand of the recipient DNA in the 3' exon. After DNA cleavage, retrohoming occurs by a target DNA-primed reverse transcription of the intron RNA that had reverse spliced into the sense strand of the recipient DNA. It also contributes to the recognition of the DNA target site and acts as a repressor of its own translation.
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