Difference between revisions of "Part:BBa K2170001:Design"
(→Design Notes) |
|||
Line 9: | Line 9: | ||
'''Related BioBrick:''' | '''Related BioBrick:''' | ||
− | Other versions:[https://parts.igem.org/wiki/index.php?title=Part:BBa_K2170002 BBa_K2170002: Secretory eukaryotic biotin binding receptor with single chain avidin] | + | Other versions:[https://parts.igem.org/wiki/index.php?title=Part:BBa_K2170002 BBa_K2170002: Secretory eukaryotic biotin binding receptor with single chain avidin]<br> |
Related BioBricks:[https://parts.igem.org/wiki/index.php?title=BBa_K2170003 BBa_K2170003: Biotinylated receptor with biotin acceptor peptide combined with a secretory BirA] | Related BioBricks:[https://parts.igem.org/wiki/index.php?title=BBa_K2170003 BBa_K2170003: Biotinylated receptor with biotin acceptor peptide combined with a secretory BirA] | ||
Revision as of 12:57, 19 October 2016
Secretory eukaryotic biotin binding receptor with enhanced monomeric avidin
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 576
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2432
Design Notes
Related BioBrick:
Other versions:BBa_K2170002: Secretory eukaryotic biotin binding receptor with single chain avidin
Related BioBricks:BBa_K2170003: Biotinylated receptor with biotin acceptor peptide combined with a secretory BirA
Cloning details:
Designed in RFC25
Mutation C889G to delete XbaI restriction site
Truncation upstream/downstream compared to template, ?explanation?
Quality control measures:
Test digestion using EcorRI & PstI
Sequencing using VF2
Part was partly sequenced
Backbone:
Backbone name: pSB1C3
Resistance: Cp
Corresponding part author/authors:
Source
Source:
Parts synthesized by IDT
Organism:
Genesequence derived from Escherichia coli
References
Literature references:
Database references: