Difference between revisions of "Part:BBa K1896010"
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This part constitutively expresses [[Part:BBa_K1896001|mGFPuv2]], a monomeric GFP variant optimised for excitation by UV light (360-400nm). | This part constitutively expresses [[Part:BBa_K1896001|mGFPuv2]], a monomeric GFP variant optimised for excitation by UV light (360-400nm). | ||
− | The J23119 promoter is a strong consensus promoter and expression is high enough for colonies to display visible fluorescence in daylight. | + | The J23119 promoter is a strong consensus promoter and expression is high enough for colonies to also display visible fluorescence in daylight. |
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+ | [[File:MGFPuv2_colonies.jpeg|250px|thumb|center|Colonies of <i>E. coli</i> containing BBa_K1896010 imaged under long-wave UV light.]] | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K1896010 SequenceAndFeatures</partinfo> | <partinfo>BBa_K1896010 SequenceAndFeatures</partinfo> |
Revision as of 21:42, 21 October 2016
mGFPuv2 generator
This part constitutively expresses mGFPuv2, a monomeric GFP variant optimised for excitation by UV light (360-400nm). The J23119 promoter is a strong consensus promoter and expression is high enough for colonies to also display visible fluorescence in daylight.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]