Difference between revisions of "Part:BBa K1896010"

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This part constitutively expresses [[Part:BBa_K1896001|mGFPuv2]], a monomeric GFP variant optimised for excitation by UV light (360-400nm).
 
This part constitutively expresses [[Part:BBa_K1896001|mGFPuv2]], a monomeric GFP variant optimised for excitation by UV light (360-400nm).
The J23119 promoter is a strong consensus promoter and expression is high enough for fluorescence to be readily visible in colonies.
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The J23119 promoter is a strong consensus promoter and expression is high enough for colonies to display visible fluorescence in daylight.
 
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Revision as of 12:52, 19 October 2016


mGFPuv2 generator

This part constitutively expresses mGFPuv2, a monomeric GFP variant optimised for excitation by UV light (360-400nm). The J23119 promoter is a strong consensus promoter and expression is high enough for colonies to display visible fluorescence in daylight.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]