Difference between revisions of "Part:BBa K1955008"

 
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<partinfo>BBa_K1955008 short</partinfo>
 
<partinfo>BBa_K1955008 short</partinfo>
  
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In order to prove the HA sequence which we designed can smoothly express protein, we use BL21 competent cell to express the HA sequence and detect the protein by Western blot analysis.
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In order to prove the HA(H1N1) sequence which we designed can smoothly express protein, we use BL21 competent cell to express the HA sequence and detect the protein by Western blot analysis.
  
 
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Revision as of 11:33, 19 October 2016


pSB1C3-LacI inducible Hemagglutinin(H1N1)

In order to prove the HA(H1N1) sequence which we designed can smoothly express protein, we use BL21 competent cell to express the HA sequence and detect the protein by Western blot analysis.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 232
    Illegal BamHI site found at 1938
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1868