Difference between revisions of "Part:BBa K2130006"

 
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Human codon optimised Lachnospiraceae bacterium ND2006 Cpf1 (LbCpf1)
 
Human codon optimised Lachnospiraceae bacterium ND2006 Cpf1 (LbCpf1)
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Cpf1 is an endonuclease that analogue to Cas9. To facilitate recognition of target sites by Cpf1, it needs a T-rich PAM sequence(TTTN) that is 5' of spacer.Since Cpf1 depend on different PAM sequence compared to Cas9, it increases the possibilities to target new genes. Lastly, Cpf1 endonuclease creates a staggered/ stick-end DNA double stranded breaks instead of blunt-end breaks.
  
 
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Revision as of 11:42, 24 October 2016


LbCpf1

Human codon optimised Lachnospiraceae bacterium ND2006 Cpf1 (LbCpf1)

Cpf1 is an endonuclease that analogue to Cas9. To facilitate recognition of target sites by Cpf1, it needs a T-rich PAM sequence(TTTN) that is 5' of spacer.Since Cpf1 depend on different PAM sequence compared to Cas9, it increases the possibilities to target new genes. Lastly, Cpf1 endonuclease creates a staggered/ stick-end DNA double stranded breaks instead of blunt-end breaks.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 25
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 965
    Illegal BglII site found at 1298
    Illegal BglII site found at 1868
    Illegal BglII site found at 2135
    Illegal BglII site found at 2756
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1027
    Illegal NgoMIV site found at 2026
    Illegal NgoMIV site found at 2728
    Illegal NgoMIV site found at 3717
    Illegal NgoMIV site found at 3736
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1356
    Illegal SapI.rc site found at 1416