Difference between revisions of "Part:BBa K1991000"

Revision as of 05:53, 19 October 2016

AOX1 (Alcohol oxidase)

Alcohol oxidase (AOX) is also an oxido-reductase that catalyzes the oxidation of alcohol and produce aldehyde and hydrogen peroxide. The substrates of AOX are primary alcohols including methanol and some short-chain alcohols such as ethanol. AOX is present in the methylotrophic yeast like Pichia pastoris, which has two genes, AOX1, AOX2 under a strong methanol inducible promoter. The yeast uses AOX genes to metabolize methanol as a carbon and energy source. The AOX enzymes were used in biosensors (Biosens Bioelectron. 2005) but with a limited application because its electrochemical properties including reduction potential are not well studied.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1687

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 <partinfo>BBa_K1991000 short</partinfo>
-There are two Alcohol oxidase (AOX) genes, AOX1 (CAY72092.1) and AOX2 (CAY71377.1), on chromosome 4 in Pichia pastoris. AOX was utilized to provide carbon source and energy for the methylotrophic yeast by metabolizing primary alcohol like methanol. 111
+Alcohol oxidase (AOX) is also an oxido-reductase that catalyzes the oxidation of alcohol and produce aldehyde and hydrogen peroxide. The substrates of AOX are primary alcohols including methanol and some short-chain alcohols such as ethanol. AOX is present in the methylotrophic yeast like Pichia pastoris, which has two genes, AOX1, AOX2 under a strong methanol inducible promoter. The yeast uses AOX genes to metabolize methanol as a carbon and energy source. The AOX enzymes were used in biosensors (Biosens Bioelectron. 2005) but with a limited application because its electrochemical properties including reduction potential are not well studied.
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