Difference between revisions of "Part:BBa K2074028"
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[[File:2016FAFUCHINADevice5.png|800px|thumb|center|'''Fig.1''' Cry4Aa and Cyt2 Toxins Group]]
 
[[File:2016FAFUCHINADevice5.png|800px|thumb|center|'''Fig.1''' Cry4Aa and Cyt2 Toxins Group]]
 +
[[File:T--FAFU-CHINA--4+2.png|600px|thumb|center|'''Figure.2''' The gel electrophoresis result of Cry4Aa+FMDV 2A+Cyt2]]
 
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===Usage and Biology===
 
===Usage and Biology===

Revision as of 19:20, 19 October 2016


Cry4Aa(Codon optimization)+Extended FMDV 2A+Cyt2(Codon optimization) 800px-T--FAFU-CHINA--partsclones.png

Cry4Aa and Cyt2 are proteins from Bacillus thuringiensis (Bt.) which have toxicity to the larvae of mosquitoes.We used 2A system to express two proteins at the same time in Chlamydomonas reinhardti. These two proteins express at the same time can enhance the toxicity to the larvae of mosquitoes.

Fig.1 Cry4Aa and Cyt2 Toxins Group
Figure.2 The gel electrophoresis result of Cry4Aa+FMDV 2A+Cyt2

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 986
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1378
    Illegal NgoMIV site found at 3631
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 31
    Illegal BsaI.rc site found at 184
    Illegal BsaI.rc site found at 2071
    Illegal BsaI.rc site found at 2611
    Illegal BsaI.rc site found at 2920
    Illegal BsaI.rc site found at 3487
    Illegal BsaI.rc site found at 4150
    Illegal SapI.rc site found at 1188
    Illegal SapI.rc site found at 1230