Difference between revisions of "Part:BBa K2100015"
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K2100015 short</partinfo>
 
<partinfo>BBa_K2100015 short</partinfo>
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pDEST-mCherry is a destination vector which contains the mCherry gene, a monomeric fluorescent protein which emits red light upon excitation. The construct is used as a negative selection marker to recognize colonies of bacteria which have plasmids that did not successfully undergo the LR reaction and remain as destination vectors.
 
pDEST-mCherry is a destination vector which contains the mCherry gene, a monomeric fluorescent protein which emits red light upon excitation. The construct is used as a negative selection marker to recognize colonies of bacteria which have plasmids that did not successfully undergo the LR reaction and remain as destination vectors.
  

Revision as of 02:40, 19 October 2016


pDEST_mCherry_GG

pDEST-mCherry is a destination vector which contains the mCherry gene, a monomeric fluorescent protein which emits red light upon excitation. The construct is used as a negative selection marker to recognize colonies of bacteria which have plasmids that did not successfully undergo the LR reaction and remain as destination vectors.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2466
    Illegal XbaI site found at 2493
    Illegal XbaI site found at 2797
    Illegal SpeI site found at 2054
    Illegal PstI site found at 1907
    Illegal PstI site found at 2505
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2466
    Illegal SpeI site found at 2054
    Illegal PstI site found at 1907
    Illegal PstI site found at 2505
    Illegal NotI site found at 971
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2466
    Illegal BamHI site found at 2487
    Illegal XhoI site found at 1031
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2466
    Illegal XbaI site found at 2493
    Illegal XbaI site found at 2797
    Illegal SpeI site found at 2054
    Illegal PstI site found at 1907
    Illegal PstI site found at 2505
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2466
    Illegal XbaI site found at 2493
    Illegal XbaI site found at 2797
    Illegal SpeI site found at 2054
    Illegal PstI site found at 1907
    Illegal PstI site found at 2505
    Illegal NgoMIV site found at 3360
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 2749
    Illegal BsaI site found at 4337
    Illegal BsaI.rc site found at 2092