Difference between revisions of "Part:BBa K2150113"
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[[file:T--UCAS--RGP_Ptet.png|500px]] | [[file:T--UCAS--RGP_Ptet.png|500px]] | ||
| − | This construction is also used to identify the interaction between toxins and antitoxins. | + | This construction is also used to identify the interaction between toxins and antitoxins. See: |
| + | [https://parts.igem.org/Part:BBa_K2150117 BBa_K2150117] | ||
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Latest revision as of 03:45, 20 October 2016
toxin ParE2 induced by aTc
Usage and Biology
This part is used to build a kill-switch.
Characterization
Growth Curve
We measured the growth curve of bacteria expressing toxin ParE2.
The growth curve of wild type E. coli harboring empty vector is shown below.
This construction is also used to identify the interaction between toxins and antitoxins. See: BBa_K2150117
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 455
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]