Difference between revisions of "Part:BBa K2150110"
 
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[[file:T--UCAS--RGP_Ptac.png|500px]]
 
[[file:T--UCAS--RGP_Ptac.png|500px]]
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Note that when no IPTG is added, there is no leakage in experimental group.
  
 
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Latest revision as of 01:53, 19 October 2016

toxin N-acetyltransferase GCN5 induced by IPTG

Usage and Biology

This part is used to build a kill-switch.

Characterization

Growth Curve

We measured the growth curve of bacteria expressing toxin N-acetyltransferase GCN5.

T--UCAS--1204t-IPTG.png

The growth curve of wild type E. coli harboring empty vector is shown below.

T--UCAS--RGP Ptac.png

Note that when no IPTG is added, there is no leakage in experimental group.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1611
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1857
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1611
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1611
    Illegal NgoMIV site found at 1788
  • 1000
    COMPATIBLE WITH RFC[1000]