Difference between revisions of "Part:BBa K1941001:Design"

(→‎Design Notes)
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===References===
 
===References===
 +
- CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes, L.A. Gilbert et al., Cell 2013.
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- Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds, J.G. Zalatan et al., Cell 2015.

Revision as of 21:26, 18 October 2016


scTef1_PP7


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The overall structure of the scRNA was taken form "Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds" J.G.Zalatan et al., Cell 2015, but the 20 base pairs specificity sequence was replaced with a sequence targeting TEF1 from "CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes", L.A. Gilbert et al., Cell 2013.

Source

some source

References

- CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes, L.A. Gilbert et al., Cell 2013. - Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds, J.G. Zalatan et al., Cell 2015.