Difference between revisions of "Part:BBa K2065002:Experience"
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===Applications of BBa_K2065002===
 
===Applications of BBa_K2065002===
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This construct was successfully created at DNA level and after this the protein was expressed. After this we did several assays with the protein, which functioned as scaffold for other proteins.
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iGEM TU Eindhoven created this scaffold protein by computational design and checked its functioning and orthogonality using the NanoBiT reporter system. This was accomplished by linking CT52, the protein which has affinity to the T14-3-3 and complementary mutant form to parts of this reporter system
  
 
===User Reviews===
 
===User Reviews===

Revision as of 20:05, 18 October 2016


Applications of BBa_K2065002

iGEM TU Eindhoven created this scaffold protein by computational design and checked its functioning and orthogonality using the NanoBiT reporter system. This was accomplished by linking CT52, the protein which has affinity to the T14-3-3 and complementary mutant form to parts of this reporter system

User Reviews

UNIQ5b5f2ab8c1142f3a-partinfo-00000000-QINU UNIQ5b5f2ab8c1142f3a-partinfo-00000001-QINU