Difference between revisions of "Part:BBa K2040101:Design"
Chilam Poon (Talk | contribs) (→Design Notes) |
Chilam Poon (Talk | contribs) (→Design Notes) |
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===Design Notes=== | ===Design Notes=== | ||
| − | One illegal | + | One illegal cutsite (PstI) were subsequently removed via site-directed mutagenesis. |
===Source=== | ===Source=== | ||
Revision as of 18:03, 18 October 2016
Fungal promoter PgpdA
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 903
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 137
Illegal XhoI site found at 693
Illegal XhoI site found at 1075 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1282
Illegal AgeI site found at 1430 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1109
Illegal SapI site found at 315
Design Notes
One illegal cutsite (PstI) were subsequently removed via site-directed mutagenesis.
Source
It was taken from vector pAN7-1.