Difference between revisions of "Part:BBa K1943007"
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For detailed characterization data, see the experience page. | For detailed characterization data, see the experience page. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | [[File:109&119.jpeg|center]] | ||
| + | Right is <partinfo>BBa_K1943004</partinfo>(Strong Promoter & RBS), Left is <partinfo>BBa_K1943005</partinfo>(Weak Promoter & RBS) | ||
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| + | [[File:SUSTech_Pattern_of_chromoprotein_1.jpeg]] | ||
| + | [[File:SUSTech_Shenzhen-SUSTecho.png|center]] | ||
| + | A few of the chromoproteins form the pattern of S(<partinfo>BBa_K1943000</partinfo>), U(<partinfo>BBa_K1943002</partinfo>), S(<partinfo>BBa_K1943004</partinfo>), T(<partinfo>BBa_K1943006</partinfo>), e(<partinfo>BBa_K1943005</partinfo>), c(<partinfo>BBa_K1943003</partinfo>), h(<partinfo>BBa_K1943001</partinfo>), !(<partinfo>BBa_K1943007</partinfo>)<br><br> | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 19:58, 19 October 2016
spisPink, Pink chromoprotein reporter system (medium Promoter , weak RBS)
Team Uppsala 2012 chromoprotein attracts many interests because it's more convenient than fluorescent protein for it can be observed with naked eyes. However, characterized data of these proteins are few. Because a single coding region is on the plasmid, we constructed BBa_K1943007 with chromoprotein spisPink(BBa_K1033932) this year and did characterization. We've constructed a series of plasmids which are all in the same pattern: a strong/weak promoter, a strong/weak RBS and a chromoprotein. We want to monitor the speed of expression of these plasmids in normal incubation conditions (like 37℃ overnight for LB agar plate and 37℃ 180rpm for LB broth). The expression speed and strength of the constructed biobricks will be carefully monitored and gave others a relative scale for using chromoprotein as a reporter gene. For detailed characterization data, see the experience page.
Usage and Biology
Right is BBa_K1943004(Strong Promoter & RBS), Left is BBa_K1943005(Weak Promoter & RBS)
A few of the chromoproteins form the pattern of S(BBa_K1943000), U(BBa_K1943002), S(BBa_K1943004), T(BBa_K1943006), e(BBa_K1943005), c(BBa_K1943003), h(BBa_K1943001), !(BBa_K1943007)
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]