Difference between revisions of "Part:BBa K1926002:Design"
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===Source=== | ===Source=== | ||
Revision as of 16:27, 18 October 2016
A cyclic promoter of Ki-67 from human genome
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 425
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 422
Design Notes
We got the sequence from human genome through PCR using the following primers:
pKi67-F: ACCTCTGCCCTCCGCCAGCCG
pKi67-R: ACCCGGTGGCCCTACAGGCTACG
(Product: 360)
Details are all at the main page.
Source
The sequence was retrieved from Addgene.
References
1. Pei, D.S., et al., Analysis of human Ki-67 gene promoter and identification of the Sp1 binding sites for Ki-67 transcription. Tumour Biol, 2012. 33(1): p. 257-66.